Hyperbaric oxygen treatment on keloid tumor immune gene expression / 中华医学杂志(英文版)

BACKGROUND@#Hyperbaric oxygen treatment (HBOT) has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids. To explore the mechanism of the effect of HBOT on keloids, tumor immune gene expression and immune cell infiltration were studied in this work.@*METHODS@#From February 2021 to April 2021, HBOT was carried out on keloid patients four times before surgery. Keloid tissue samples were collected and divided into an HBOT group (keloid with HBOT before surgery [HK] group, n = 6) and a non-HBOT group (K group, n = 6). Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit. Data were mined with R package. The differentially expressed genes between the groups were compared. Hub genes between the groups were determined and verified with Quantitative Real-time PCR. Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry (IHC).@*RESULTS@#Inflammatory cell infiltration was reduced in the HK group. There were 178 upregulated genes and 217 downregulated genes. Ten hub genes were identified, including Integrin Subunit Alpha M (ITGAM), interleukin (IL)-4, IL-6, IL-2, Protein Tyrosine Phosphatase Receptor Type C (PTPRC), CD86, transforming growth factor (TGF), CD80, CTLA4, and IL-10. CD80, ITGAM, IL-4, and PTPRC with significantly downregulated expression were identified. IL-10 and IL-2 were upregulated in the HK group but without a significant difference. Infiltration differences of CD8 lymphocyte T cells, CD4 lymphocyte T-activated memory cells, and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis. Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.@*CONCLUSION@#HBOT affected tumor gene expression and immune cell infiltration in keloids. CD4 lymphocyte T cell, especially activated memory CD4+T, might be the key regulatory immune cell, and its related gene expression needs further study.

Resection and repair of large abdominal wall lesions in gynecologic patients / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The techniques of resection and repair of large lesions in the abdominal wall are very challenging in the area of gynecology. We explored the techniques of resection and plastic surgical repair of large abdominal wall lesions in gynecologic patients.</p><p><b>METHODS</b>Twenty-six patients with large lesions in the abdominal wall underwent resection by the gynecologists and repair through abdominal plasty and V-Y plasty with or without fascia patch grafting by the gynecologists or plastic surgeons from March 2003 to October 2010.</p><p><b>RESULTS</b>All patients had a history of cesarean section. One patient had an infected sinus tract after cesarean section, one patient had an inflammatory nodule, and the others had lesions of endometriosis, including one cancer. The average largest lesion diameter was (4.79 ± 4.18) cm according to the ultrasonography results. The lesions of all patients were completely resected with pretty abdominal contour. A polypropylene biological mesh was added to the fascia in 20 patients. One patient underwent groin flap repair, and one underwent V-Y advanced skin flap repair on the left of the incision to relieve the suture tension.</p><p><b>CONCLUSIONS</b>Multi-department cooperation involving the gynecology and plastic surgery departments, and even the general surgery department, is essential for patients with large lesions in the abdominal wall. This cooperative effort enabled surgeons to completely resect large lesions. Abdominal wall plastic surgical repair can ameliorate large wounds of the abdominal wall.</p>

Mini-abdominoplasty combined with mesh used for abdominal wall endometriosis / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Wide excision is considered the treatment of endometriosis. It is difficult to surgeon for reconstruction of a large full-thickness defect through the abdominal-wall. We introduce a method of mini-abdominoplasty combined with mesh that can be used for reconstruction of a large full-thickness defect through the abdominal-wall after wide excision of abdominal wall endometriosis.</p><p><b>METHODS</b>This retrospective study includes a series of patients who underwent wide excision of abdominal wall endometriosis and reconstruction of a large full-thickness defect through the abdominal-wall over a 5-year period. Information obtained from chart reviews includes age, size of lesion and defect, complications and revisions.</p><p><b>RESULTS</b>The method was used for 8 patients including 2 patients with recurrence. The mean size of the masses was (3.5 ± 2.0) cm. The mean size of the fascia defects was 7.1 cm × 8.6 cm. The mean length of follow-up was (24 ± 12) months. There was no recurrence, no hernia, and no other complications. The technique generated only a horizontal scar. The scar and contour of the lower abdomen provided a more pleasant appearance than the traditional procedure.</p><p><b>CONCLUSIONS</b>Mini-abdominoplasty combined with mesh is a useful and acceptable reconstruction method for large full-thickness defects through the abdominal wall after endometriosis resection. It is feasible for wide excision with 1 cm normal tissues around the margin. It provides an aesthetically pleasing result.</p>

Site-specific pathology in breast reduction mammoplasty / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the feasibility and clinical value of site-specific pathology in breast reduction mammoplasty.</p><p><b>METHODS</b>Totally 127 patients who underwent breast reduction mammoplasty from June 2007 to June 2010 were included. Pathologic specimens were sent for frozen pathology according to the clock-wise method. Patients with confirmed cancer were arranged for conservative breast surgery.</p><p><b>RESULTS</b>Of the 127 patients, 53 patients (41.7%) had gland hyperplasia; 7 had fibroadenoma (5.5%); 1 (0.79%) had ductal carcinoma in situ, who underwent breast conserving surgery, following by chemotherapy and radiotherapy, no relapse was noted during the three-year follow-up.</p><p><b>CONCLUSION</b>Clock-wise method is useful to locate the tumor and ensure the patients to receive skin-spared breast resection even cancer is detected.</p>

Application of melanocytes and bone marrow mesenchymal stem cells in tissue engineered skin construction / 中国医学科学院学报

<p><b>OBJECTIVE</b>To explore the method of constructing tissue-engineered skin using melanocytes and bone marrow mesenchymal stem cells (BMSCs) in vivo.</p><p><b>METHODS</b>Melanocytes were isolated from human foreskin. BMSCs were isolated from human bone marrow. Both of them were co-cultured at a ratio of 1:10, and then were implanted into the collagen membrane to construct the tissue-engineered skin, which was applied for wound repair in nude mice. The effectiveness of wound repair and the distribution of melanocytes were evaluated by morphological observation, in vivo 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.</p><p><b>RESULTS</b>The wounds were satisfactorily repaired among the nude mice. The melanocytes were distributed in the skin with normal structure, as confirmed by DAPI fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.</p><p><b>CONCLUSION</b>Melanocytes and BMSCs, after proper in vitro culture at an appropriate ratio, can construct the tissue-engineered skin with I type collagen membrane.</p>

Application of abdominoplasty and polypropylene to repair the abdominal defect / 中华整形外科杂志

<p><b>OBJECTIVE</b>To introduce a operation method of repairing the abdominal defect with abdominoplasty and polypropylene.</p><p><b>METHODS</b>27 patients were treated with abdominoplasty and polypropylene.</p><p><b>RESULTS</b>Follow up 3 - 20 months, the focus in inferior abdominal wall were removed thoroughly. The incision is cure with A level. No weakness, bulk, hernia and complication occur in the abdominal wall.</p><p><b>CONCLUSIONS</b>The method of repairing the abdominal defect with abdominoplasty and polypropylene is a good method. It is a simple method. Its' result is relied.</p>

Analysis of Millard II technique for correcting secondary deformities of unilateral cleft lip / 中华整形外科杂志

<p><b>OBJECTIVE</b>To analyze the Millard II technique for correcting secondary deformities of unilateral cleft lip.</p><p><b>METHODS</b>The Millard II technique was used to correct secondary deformities of unilateral cleft lip in 42 patients from March of 2003 to September of 2004. Dissection was made between the alar cartilage and skin, and the alar cartilage was suspended.</p><p><b>RESULTS</b>The postoperative follow-ups with 3 approximately 6 months revealed good results of the symmetrical nostrils and philtrums, prolonged columella nasi, good-shaped cupid's bow, and invisible scar.</p><p><b>CONCLUSIONS</b>The Millard II technique could be an ideal method to correct secondary deformities of unilateral cleft lip. Releasing and suspending alar cartilage spontaneously at the same time can correct nasal deformity adequately.</p>

Repairment of the large wound of endometriosis in the abdominal wall / 中国医学科学院学报

<p><b>OBJECTIVE</b>To explore the plastic surgical repairment of the large wound of endometriosis in the abdominal wall.</p><p><b>METHOD</b>Since March 2003 to December 2004, 6 patients were treated with abdominoplasty and V-Y plasty for the wounds of the endometriosis in the abdominal wall.</p><p><b>RESULTS</b>The endometriotic foci were removed thoroughly with pretty abdominal contour. No complications were observed.</p><p><b>CONCLUSION</b>Abdominoplasty and V-Y plasty are good methods to repair the wounds of the endometriosis in the abdominal wall.</p>

Preventive effect of chitosan on capsular contracture after implantation of silicone gel-filled mammary implants in rats / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the preventive effect of chitosan on capsular contracture after implantation of silicone gel-filled mammary implants in rats.</p><p><b>METHODS</b>18 SD female rats were divided into three groups randomly. Small silicone gel-filled mammary implants were implanted under each side of latissimus dorsi muscle. Chitosan was injected into left cavities as experimental sides, and nothing was injected into right cavities as control sides. 4, 8 and 12 weeks postoperation, specimens were dissected and observed using light microscope, and area density of collagen both type I and type III were also detected.</p><p><b>RESULTS</b>Fibrous capsules around implants consist of two layers: dense layer and loose layer. 4 weeks postoperation, total thickness of the experimental sides was less than that of the control sides (P < 0.01). Area density of collagen either type I or type III of the experimental sides was lower than that of the control sides (P < 0.01). Both 8 and 12 weeks postoperation, total thickness of the experimental sides was less than that of the control sides (P < 0.05). Area density of collagen type I of the experimental sides was lower than that of the control sides (P < 0.05). Area density of collagen type III was of no statistical difference between the experimental sides and the control sides (P > 0.05).</p><p><b>CONCLUSIONS</b>Chitosan can remarkably prevent capsule contracture during the early period after implantation of silicone gel-filled mammary implants.</p>